Latest paper published : microspheres for fluorescence detection

5 Septembre 2008 , Rédigé par JW Publié dans #Recent research work

Just published in September 1 issue of Analytical Chemistry :

Disposable Microscope Objective Lenses for Fluorescence Correlation Spectroscopy Using Latex Microspheres

 

An inexpensive low numerical aperture lens is used instead of a costly conventional high NA microscope objective to detect single molecule fluorescence, thanks to a latex microsphere set at the lens focus. The graphs below show fluorescence intensity correlation functions obtained with a 0.25NA doublet and a 3 µm microsphere and reference correlation function with a standard water-immersion 1.2NA microscope objective and no microsphere.

 

We demonstrate viable FCS experiments at concentrations 1−1000 nM with different objectives costing less than $40. This offers a simple and low-cost alternative to the conventional complex microscope objectives.