Microscope Setup Views
Maybe one of the most crucial part, which will never be published in a paper ! The main picture shows the microscope at the left, the lasers at the center back, and the confocal detection at the forefront right. There are many optical parts, each has a specific role and is manually assembled and aligned.
The second picture focuses on our Zeiss axiovert 35M (inverted) microscope. I should at some time describe it into more details, maybe on a microscope enthusiasts website (micscape).
And finally the microscope objective, which is by far the central component of the setup. We’re using a Zeiss C-Apochromat with 40x magnification, 1.2 numerical aperture, water immersion, coverslip thickness compensation collar and infinite correction. I’m pretty pleased with it for single-color confocal microscopy close to the diffraction limit, but care must be taken to compensate for chromatic aberrations.