Multi-focus FCS with photonic nanojets arrays
Our article “Multi-focus parallel detection of fluorescent molecules at picomolar concentration with photonic nanojets arrays” has just been released in Applied Physics Letters. We replace the complex microscope objective commonly used in fluorescence sensing by an array of latex microspheres. This realizes a novel regime where several focal spots are illuminated to detect the possible presence of a fluorescent molecule in one of them. A detailed theoretical description of the phenomen is provided along with experimental demonstration.
This work describes an efficient disposable lens element that enables single molecule sensitivity at low picomolar concentration. The simplicity of the design makes it perfect for integration in portable microfluidic readers.