In the July issue of Nature Nanotechnology, read the News & Views about our recent work by Prof. Philip Tinnefeld.
"The combination of a plasmonic nanoantenna and a nanoaperture has merged fluorescence enhancement and spatial confinement to enable single-molecule detection at biologically relevant concentrations."
In a collaboration between the Fresnel Institute and ICFO-the Institute for Photonic Sciences, we report in Nature Nanotechnology a novel “antenna-in-box” platform for single molecule fluorescence detection with unprecedented resolutions and sensitivity. The innovative approach combines a plasmonic gap antenna for ultra-high fluorescence enhancement with a metal nanoaperture for optimized background-free operation. It allows for 1100-fold fluorescence brightness enhancement together with detection volumes down to 58 zeptoliters (1 zL = 1e-21L), realizing a gain of four orders of magnitude as compared to classical microscopes. The antenna-in-box offers a highly efficient platform for nanoscale biochemical assays with single molecule sensitivity at physiological conditions.
Submissions and registrations for the 3rd Summer School On Plasmonics are now open. This summer school will bring together international specialists in Plasmonics on one of the most beautiful islands of the Mediterranean. Check out the exciting program and the list of invited speakers at http://www.fresnel.fr/ssop3/
11 courses and 2 tutorials will be presented by international specialists on the following topics:
-Light scattering by metallic particles
-Limits of diffraction
-Dielectric permittivity of metals
-Nonlocal and quantum effects
-Quantum Plasmonics and Magnetic Metafluids
-3D optical metamaterials and transformation optics
-Optical spectroscopy of metal nanoparticles
Deep's abstract on Plasmonic nanoantennas for enhanced single molecule analysis at micromolar conentrations has just been promoted for invited talk presentation at the 6th International Conference on Surface Plasmon Photonics SPP6.
As a teaser, our main claims are:
- fluorescence enhancement up to 1100-fold AND fluorescence brightness per molecule up to 400kHz (detected)
- detection volume measured down to 58 zeptoliters, four orders of magnitude below the diffraction limit
- true single molecule detection above 20 µM concentration actually measured
- our dedicated design is fully compatible with detection of single fluorescent molecules in solution and fluorescence correlation spectroscopy