The Institut Fresnel is a research state laboratory based in Marseille / France, devoted to research and higher education in all areas of optical and photonic sciences. The Institut Fresnel is seeking to recruit talented, enthusiastic young scientists who are highly motivated to boost their research career in the areas of nano-optics and/or biophotonics. See the general call webpage here.
DONE - POSITION FILLED PhD ExtendFRET : Extended fluorescence energy transfer with nanophotonics
DONE - POSITION FILLED PhD Twins : Theoretical / numerical research in plasmonics and optical antennas
DONE - POSITION FILLED PostDoc ExtendFRET : Plasmonic circuits design & simulations
Found that e-book free online, might be of interest for PhD students / young postdocs:
Charting a Course for a Successful Research Career - 2nd edition by Prof. Alan M Johnson
As researchers operate in a progressively competitive research environment, with grant applications increasing, funding opportunities decreasing, and funding bodies tightening the reins around their requirements and expected outcomes - early career researchers are often at a disadvantage when applying for grants. In this helpful guide, Professor Alan M Johnson, steers early career researchers through the potential pitfalls and opportunities when planning for a successful research career.
Fluorescence is a powerful and versatile method that is widely used in several scientific fields. However, when it comes to analyze the origin of a given fluorescence signal (i.e. calibrating the excitation intensity, the number of emitters and their respective quantum yield), fluorescence spectroscopy is intrinsically limited, as it is unable to separately quantify the excitation intensity from the number of fluorescent emitters. This may cause severe misinterpretation of experimental results as soon as complex systems are used, such as plasmonic metal substrates or scattering media. The range of application is large, from bioassays to microscopy imaging.
We address this issue in a recent Optics Letters publication by monitoring higher-order harmonic fluorescence signals upon harmonic excitation modulation. To our knowledge, this is the very first method able to quantify the excitation intensity and the number of emitters separately. It is a significant supplement to the fluorescence toolbox. The method is compatible with a wide range of observations, and relatively simple to implement. This opens new characterization routes for applications on surface-enhanced fluorescence bioassays, microscopy across scattering samples, and deep tissue fluorescence imaging.