Following in SPIE Photonics West conference in last January, we have a proceedings paper published.
High-efficiency single molecule fluorescence detection and correlation spectroscopy with dielectric microspheres
H. Aouani, N. Djaker, J. Wenger, and H. Rigneault, Proc. of SPIE, Proc. SPIE Vol. 7571, pp.75710A (2010)
A personal reprint can be found here.
We report of the following new results :
- microspheres made of high refractive index melamine resin provide a seven-fold fluorescence enhancement on single molecules.
- this effect can be used to increase the signal-to-noise ratio in fluorescence correlation spectroscopy and reduce the experiment integration time by fifty-fold.
- first description of dual-color fluorescence cross-correlation spectroscopy (FCCS) enhanced by a microsphere.
Going back to basics, my first Micscape paper is thus devoted to an early student microscope stand of the second mid of the 19th century. Thanks to its open design, this microscope is ideal for demonstrations, and also for oblique epi-darkfield illumination to reveal physical colors from diatoms.
Abstract: Recent advances in nanophotonics open the way for promising applications towards efficient single molecule fluorescence analysis. In this review, we discuss how photonic methods bring innovative solutions for two essential questions: how to detect a single molecule in a highly concentrated solution, and how to enhance the faint optical signal emitted per molecule? The focus is set primarily on the widely used technique of fluorescence correlation spectroscopy (FCS), yet the discussion can be extended to other single molecule detection methods.