Super-resolution microscopy techniques such as PALM/STORM/STED are revolutionizing optical imaging. While diffraction is no longer a limit, another technical barrier arises driven by the need to compensate for mechanical and thermal drifts at the nanoscale. In a recent article published in Nature Communications, we introduce a novel approach to enhance the image quality in dSTORM microscopy by 3D super-localization of nanoparticles with unprecedented nanometer accuracy.
- We show how to exploit the phase response of nanoparticles to recover their 3D position with sub-nanometer accuracy, two orders of magnitude below the diffraction limit, even with large and unknown drifts of several microns.
- The platform uses a commercial wavefront sensing camera to realize highly efficient 3D stabilization and resolution improvement for super-resolution microscopes.
This work provides a leap towards the design of optical super-resolution microscopes with nanometer resolutions, reaching the dream of biological imaging at the molecular scale.
Had a verynice interaction with the audience this morning at the European Summer School on the Physics of Light in Strasbourg.
My slides can be found here: Concentrating Light at the Nanoscale, Plasmonics and Nano-Optics
Featuring some colleagues of Institut Fresnel \ Mosaic