We currently have two open positions with funding from China Scholarship Council CSC and Groupe Ecoles Centrales GEC-CSC:
- One for a PhD: "UV autofluorescence microscopy"
- One for a postdoc: "Fiber-integrated plasmonic nanotweezers to manipulate single proteins"
Find them on the https://gec-csc.fr/ website with application form, section Natural & Life sciences: Physics & Astronomy. Application deadline January 10 2023!
It is not mentioned on the application website but I would expect Chinese nationality is a requirement for eligibility.
We're hiring! PhD fellowship on dynamic control in hybrid plasmonic nanopores: road to next generation multiplexed single molecule detection
Within the Marie Skłodowska-Curie Doctoral Networks DYNAMO merging of 6 world-leading academic groups and 1 high tech company, we are offering one full PhD fellowship on plasmonic trapping and enhanced UV label-free single protein detection with plasmonic nanopores.
See the complete details in the description enclosed below. The application website is here.
You may also find it on Academic Positions
• Regular submission deadline: September 6, 2022
• Regular registration deadline: October 4, 2022
• Conference: Oct 25-27, 2022
Effective summary: seeing the intrinsic emission from a single natural protein with ultraviolet optical antennas
One of the ultimate goals of molecular biology is to watch how single proteins work in their native state. The current mainstream approach of single molecule fluorescence relies on introducing external fluorescent markers which can lead to severe issues affecting the experimental results. As an alternative to fluorescence labelling, working in the ultraviolet is appealing to take advantage of the intrinsic autofluorescence naturally present in the vast majority of proteins. However, proteins are orders of magnitude dimmer as compared to conventional fluorescent dyes, so that single protein UV detection has remained a challenge so far. New nanotechnology tools need to be introduced to meet this challenge.
In a recent publication in Nature Communications Ultraviolet optical horn antennas for label-free detection of single proteins, our team introduces a novel optical horn antenna platform for label-free detection of single proteins in the UV with unprecedented resolutions and sensitivity. The approach combines (i) a conical horn reflector for fluorescence collection at ultrahigh angles with (ii) a metal nanoaperture for fluorescence enhancement and background screening. Real-time detection of UV autofluorescence from immobilized and diffusing single label-free proteins is demonstrated, together with experiments monitoring unfolding and dissociation upon denaturation of a widely used protein with single molecule resolution.
Optical horn antennas open up a unique new form of spectroscopy enabling the investigation of single proteins in their native state and in real time. This work provides a leap towards the design of biochemical assays with label-free single protein resolution as well as bright optical nanosources.
Improving single molecule fluorescence detection with metal zero-mode waveguide nanoapertures: recent developements
Jerome will give an invited talk at the Biophysical Society Meeting next week. For those unable to join or for those just curious about it, here is a copy of the presentation slides about using metal nanoapertures to improve single molecule fluorescence detection.