Jerome Wenger NanoBioPhotonics

The Instrumentation Prize from the Chemical Physics / Physical Chemistry division of the French Chemical and Physical Societies acknowledges the development of a new technology for chemical detection. This year, the prize has been awarded to Stéphanie Vial and Jérome Wenger for their PhoCCS technology: high sensitivity single-step biomolecular detection system from dual color nanoparticle scattering. Warm thanks to the committee and sponsors!

Book your seats for the upcoming shows:

- Ircelyon scientific day, Lyon, January 29

- Fluorescence markers for advanced microscopy, Les Houches, March 18-23

- Gold 2018 international conference, Paris, July 15-18

- NFO-15 international conference of Near-field Optics and Nanophotonics, Troyes, August 26-31

- Picoquant single molecule workshop, Berlin, September 12-14

Comment from a friend: “your rockstar analogies are nice, but you don’t have a groupie…” Hmm, that’s something I certainly need to be working on :)

You can find it on the TEL HAL repository here.

Plasmonic nanoantennas have a high potential to advance biological studies at the nanoscale. However, their use has been almost entirely focused on enhancing the fluorescence signal from individual molecules. In a recent Nano Letters paper entitled “Planar optical nano-antennas resolve cholesterol-dependent nanoscale heterogeneities in the plasma membrane of living cells”, we present the first report of optical nanoantenna arrays to study the diffusion dynamics of lipids in the membrane of living cells at the nanoscale. Our approach combines advanced nanofabrication routes with single molecule fluorescence studies using fluorescence burst analysis and fluorescence correlation spectroscopy.

Significance:

- We prove the existence of highly transient and fluctuating nanoscale assemblies of sterol and sphingolipids in living cell membranes. These nanodomains are cholesterol dependent and directly relate to the topic of lipid rafts, which has been heavily debated in the biological community for the last two decades.

- We establish a general methodology using nanoantennas to probe the architecture of live cell membranes with unprecedented spatial and temporal resolutions. This constitutes a major breakthrough in our ability to address the dynamics and interactions of molecules on the plasma membrane of living cells.

Access through Nano Letters webpage, also available on arXiv and Researchgate.

Congratulations to Dr Regmi for his brilliant defence!